NanoString
NanoString nCounter SPRINT Profiler
The nanoString nCounter SPRINT Profiler allows researchers to digitally examine multiple RNA, DNA pathways in a single tube in an extraction-free workflow. It is compatible with difficult sample types, including FFPE tissue.
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Platform Specifications
- Support for total RNA, cell lysates in GITC, FFPE-derived total RNA, and PAXgene-lysed whole blood.
- Multiplexing of up to 800 targets.
- Up to 35 µl reaction volume.
- 15 zeptomole spike-in control.
- Linear dynamic range of up to 6 x 10⁵ total counts.
- Throughput of 12 lanes per six hours.
NanoString GeoMX Digital Spatial Profiler
The GeoMX Digital Spatial Profiler - Nanostring allows for whole tissue sections, FFPE or fresh frozen to be imaged and stained for RNA or protein. ROI mapping directs the results to your analyzer while showing where in your sample the data originated. This will allow researchers to select which tissue compartments or cell types to profile based on biology. Samples run will be undamaged and may be reused.
Expression levels can be counted using the nCounter Analysis System.
The GeoMx Digital Spatial Profiler (DSP) provides morphological context in spatial transcriptomics and spatial proteomics experiments from just one slide.
Scheduling and Training
Forms
All Principle Investigators (PI) must complete the User Agreement Form before using any Research Core equipment.
Pricing Schedule
Click Here to view our current rate sheet. Contact Mary Hamer with any questions on specific equipment.
Assisted support is available for some instruments at an additional cost.
Scheduling
To schedule on equipment, contact Mary Hamer at SOMResearchCore@medsch.ucr.edu
Safety training is required for all instruments and will be addressed after the project form has been submitted and accepted.
Training
All potential users are required to complete the General SOM Training Modules.
Upon completion of all training modules, please send a copy of the transcript to SOMResearchCore@medsch.ucr.edu. After transcript review, you will be contacted to schedule a hands-on training session or validation.
Notification of access to the facility will be sent to both the PI and the user.