Cell Sorter and Flow Cytometry Core Facility
MoFlo Astrios EQ Cell Sorter
The MoFlo Astrios EQ Cell Sorter offers state-of-the-art capabilities in flow cytometry applications and isolation of specific cell populations. The sorter is equipped with the following lasers:
- 488 nm blue laser with 5-PMT detectors + Forward Scatter & Side Scatter
- 405 nm violet laser with 2-PMT detectors
- 561 nm yellow laser with 3-PMT detectors
- 640 nm red laser with 3-PMT detectors
Download the Cell Sorter Project Form:
Training is not available for the cell sorter.
Key Features
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Key Features of the MoFlo Astrios EQ Cell Sorter
Key Features
With this configuration, users may perform a wide variety of applications with standard blue, red, and violet panels. In addition, the 561 nm yellow laser opens up capability for gene expression applications by providing the ability to use a variety of “fruit proteins” as expression markers. The system has 75 and 100 micron nozzles to manage most cell types, with the ability to add additional nozzles up to 200 microns to meet most experimental demands to handle a wide range of cell types. It also has sample agitation and temperature control, to enhance cell viability and throughput.
The advantage of jet-in-air sorters on post-sort cellular viability will be useful for cell based assays, cell transplantation, and long term cell cultures. In addition, the ability to use high pressure and higher sort rates, up to 70,000 events per second, will improve the capability of efficiency of sorts that demand millions of events.
This cell sorter includes the MoFlo Astrios Clonal Plate Deposition System for applications such as cloning or screening into 96-, 384, and 1536-well plates and other formats. The Astrios offers 6-way sorting and ensures sorting efficiency, and will be contained in a Biosafety Cabinet to provide assured biological safety level 2 (BSL2) containment without compromising workflow.
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NovoCyte Quanteon Flow Cytometer Systems 4 Lasers
The NovoCyte Quanteon is a 4 laser flow cytometer with configuration for up to 25 independent photomultipliers. The system provides sensitivity, stability, and a 7.2 log dynamic range allowing dim and bright signals to be captured and gated in the same view.
Large color panels can be developed in shorter setup and analysis times via steady and consistent sample delivery. The NovoCyte Q has high reproducibility and low CVs. Absolute counts without beads can save time and money.
The NovoCyte Q software is user-friendly and easy to learn. Data and samples from the flow cytometer can be applied to the Astrios for more comprehensive sorting, as needed.
Scheduling and Training
Forms
All Principle Investigators (PI) must complete the User Agreement Form before using any Research Core equipment.
Pricing Schedule
Click Here to view our current rate sheet. Contact Mary Hamer with any questions on specific equipment.
Assisted support is available for some instruments at an additional cost.
Scheduling
To schedule on equipment, contact Mary Hamer at SOMResearchCore@medsch.ucr.edu
Safety training is required for all instruments and will be addressed after the project form has been submitted and accepted.
Training
All potential users are required to complete the General SOM Training Modules.
Upon completion of all training modules, please send a copy of the transcript to SOMResearchCore@medsch.ucr.edu. After transcript review, you will be contacted to schedule a hands-on training session or validation.
Notification of access to the facility will be sent to both the PI and the user.