Multiphoton Core Facility
The Nikon A1R MP Plus is a multiphoton confocal microscope that provides optimum imaging configurations for brain research, other neuroscience applications and in vivo imaging of living specimens.
Lab groups may advance their research and work with the technician to learn to properly use the equipment and the Nikon Elements software, and to tailor to each groups’ needs.
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Key Elements of the Nikon A1R MP Plus System
- Gallium-arsenide-phosphide (GaAsP) non-descanned detectors (NDD), which detect highly scattered multiphoton excitation and are twice as sensitive as standard NDDs, allowing for deep tissue imaging.
- An auto-aligned tunable laser (700-1080 nm), which reduces user risk and allows faster acquisition.
- A spectral detector that has 32 channels and a 2.5 nm, 6 nm, and 10 nm grating wavelength resolution; therefore, a spectra over a range of 320 nm may be collected. This also includes linear unmixing capabilities to remove any unwanted spectra.
- A hybrid scanner, including a high-resolution Galvano scanner, which uses a mechanical mirror mechanism to capture fine sample details covering a wide angle at 10 frames per second (fps), and an ultrahigh-speed resonant scanner, which is the fastest in the world (able to image at 420 fps). Although resonant scanner images are noisier, Nikon Elements software can smooth out these images, making the resonant scanner ideal for experiments that need quick image capture.
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Nikon Elements Software Manual
- Watch the Nikon A1R Training Video on YouTube
UCR SOM Multiphoton Microscope Samples
Scheduling and Training
Forms
All Principle Investigators (PI) must complete the User Agreement Form before using any Research Core equipment.
Pricing Schedule
Click Here to view our current rate sheet. Contact Mary Hamer with any questions on specific equipment.
Assisted support is available for some instruments at an additional cost.
Scheduling
To schedule on equipment, contact Mary Hamer at SOMResearchCore@medsch.ucr.edu
Safety training is required for all instruments and will be addressed after the project form has been submitted and accepted.
Training
All potential users are required to complete the General SOM Training Modules.
Upon completion of all training modules, please send a copy of the transcript to SOMResearchCore@medsch.ucr.edu. After transcript review, you will be contacted to schedule a hands-on training session or validation.
Notification of access to the facility will be sent to both the PI and the user.